{"id":176308,"date":"2021-08-25T08:24:46","date_gmt":"2021-08-25T12:24:46","guid":{"rendered":"https:\/\/today.uconn.edu\/?p=176308"},"modified":"2021-10-04T11:31:31","modified_gmt":"2021-10-04T15:31:31","slug":"uconn-researchers-identify-pathogens-infecting-cannabis-plants-in-ct","status":"publish","type":"post","link":"https:\/\/today.uconn.edu\/2021\/08\/uconn-researchers-identify-pathogens-infecting-cannabis-plants-in-ct\/","title":{"rendered":"UConn Researchers Identify Pathogens Infecting Cannabis Plants in CT"},"content":{"rendered":"<p>Cannabis has become an important part of Connecticut\u2019s economy. With the recent <a href=\"https:\/\/portal.ct.gov\/cannabis\/?language=en_US\">legalization<\/a> of adult recreational marijuana use, it is only expected to continue growing.<\/p>\n<p>This means it\u2019s important to make sure cannabis plants themselves are also, literally, growing, as well as they can.<\/p>\n<p>Ph.D. candidate Cora S. McGehee and associate professor of horticulture Rosa E. Raudales from the <a href=\"https:\/\/cahnr.uconn.edu\">College of Agriculture, Health and Natural Resources<\/a> published a study in <a href=\"https:\/\/www.frontiersin.org\/articles\/10.3389\/fagro.2021.706138\/full?utm_source=F-NTF&amp;utm_medium=EMLX&amp;utm_campaign=PRD_FEOPS_20170000_ARTICLE\"><em>Frontiers in Agronomy<\/em><\/a> documenting the presence of pathogens infecting cannabis plants in Connecticut. This was the first paper that showed these pathogens are present in Connecticut\u2019s cannabis growing facilities.<\/p>\n<p>Plants of all kinds, including cannabis, suffer from infections by a variety of pathogens. So, McGehee and Raudales stumbled into this study by accident. McGehee\u2019s research focuses on plant-microbe interactions in relation to root diseases caused by pathogenic<em> Pythium<\/em> species in hydroponic lettuce. But her training and Raudales\u2019 expertise in plant pathology put them in a unique position to address similar concerns in a new, high potential crop.<\/p>\n<p>\u201cIt\u2019s unavoidable with all the cannabis production around us,\u201d McGehee says.<\/p>\n<p>They were called in on a site visit to assess overall plant health on some cannabis plants in a Connecticut facility. However, during their visit the researchers quickly realized some of the plants were symptomatic of root rot, a disease that causes yellowing, wilting, and reduced growth.<\/p>\n<p>From there, the researchers\u2019 curiosity took over as they tried to get to the bottom of this disorder infecting these plants.<\/p>\n<p>The researchers took soilless substrate samples from coconut coir and rockwool the plants were growing in and performed a series of analyses and experiments. They took samples from the seedling stage as well as mature plants.<\/p>\n<p>First, they looked at the morphological characteristics of the organisms obtained from the soilless substrates. By looking at the size and shape of the sexual and asexual structures under a microscope, the researchers were able to identify what kind of organisms they were looking at.<\/p>\n<p>From there, they isolated DNA from the organisms, amplified genes specific to the organisms, and sent the amplified products to Yale University and Eurofins Genomics LLC laboratories for sequencing which allowed McGehee and Raudales to match their organisms sequences against a national database containing other researchers\u2019 sequences to confirm their identifications.<\/p>\n<p>The researchers identified one isolate of <em>Fusarium oxysporum<\/em>, three isolates of <em>Globisporangium<\/em> <em>irregulare<\/em> and 21 isolates of <em>Pythium myriotylum<\/em>. Aside from being the most abundant pathogenic species in the samples, <em>Pythium myriotylum<\/em> isolates were also the most virulent, or harmful, pathogens.<\/p>\n<p>\u201cOnce infected, it\u2019s very difficult to revive the plant and use it for profit,\u201d McGehee says. \u201cIt\u2019s pretty much a goner.\u201d<\/p>\n<p>The pair then conducted pathogenicity assays with hemp in the laboratory and greenhouse. They set up hundreds of hemp plants in UConn\u2019s Plant Science Research greenhouse and intentionally infected them with the pathogens.<\/p>\n<p>After monitoring these plants carefully, checking daily for symptoms the researchers confirmed all the species and isolates they tested were pathogenic to cannabis plants.<\/p>\n<p>Identifying pathogens of concern provides cannabis growers with important information. By knowing which pathogens \u201clike\u201d cannabis plants, growers can adopt more effective prevention strategies that specifically target these pathogens.<\/p>\n<p>For instance, <em>Pythium<\/em> species thrive in wet environments. This means growers should be sure to avoid overwatering their crops.<\/p>\n<p>These pathogens also spread easily, meaning growers need to remove infected plants and debris as soon as possible to prevent the pathogen from infecting other crops in production.<\/p>\n<p>The study also found the pathogens were sensitive to mefenoxam, the active ingredient in chemical fungicides used to suppress Pythium. However, this chemical is not labeled for use on cannabis crops.<\/p>\n<p>Growers can employ other strategies such as monitoring water use, pH, and soluble salt levels as well as cleaning, sanitizing, and using biological fungicides to mitigate the impact of these pathogens.<\/p>\n<p>\u201cIt\u2019s important for growers to start matching these diseases with the pathogens that cause them,\u201d McGehee says. \u201cI think that will help management strategies and prevent disease outbreaks in their facilities.\u201d<\/p>\n<p><em>\u00a0<\/em><\/p>\n<p><em>Learn more about CAHNR\u2019s <\/em><a href=\"https:\/\/cannabis.cahnr.uconn.edu\"><em>cannabis programs<\/em><\/a><em>. Follow <\/em><a href=\"https:\/\/linktr.ee\/uconncahnr_social\"><em>UConn CAHNR<\/em><\/a><em> on social media<\/em><\/p>\n","protected":false},"excerpt":{"rendered":"<p>The study showed and helped address the presence of Pythium pathogens in Connecticut\u2019s cannabis growing facilities<\/p>\n","protected":false},"author":127,"featured_media":176309,"comment_status":"closed","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"_acf_changed":false,"_crdt_document":"","wds_primary_category":0,"wds_primary_series":0,"wds_primary_attribution":0,"footnotes":""},"categories":[2354,2224,2303,2076,2235],"tags":[],"magazine-issues":[],"coauthors":[2140],"class_list":["post-176308","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-cannabis","category-cahnr","category-plant-science-landscape-architecture","category-research","category-today-homepage"],"pp_statuses_selecting_workflow":false,"pp_workflow_action":"current","pp_status_selection":"publish","acf":[],"publishpress_future_action":{"enabled":false,"date":"2026-04-25 07:40:22","action":"change-status","newStatus":"draft","terms":[],"taxonomy":"category","extraData":[]},"publishpress_future_workflow_manual_trigger":{"enabledWorkflows":[]},"_links":{"self":[{"href":"https:\/\/today.uconn.edu\/wp-rest\/wp\/v2\/posts\/176308","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/today.uconn.edu\/wp-rest\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/today.uconn.edu\/wp-rest\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/today.uconn.edu\/wp-rest\/wp\/v2\/users\/127"}],"replies":[{"embeddable":true,"href":"https:\/\/today.uconn.edu\/wp-rest\/wp\/v2\/comments?post=176308"}],"version-history":[{"count":1,"href":"https:\/\/today.uconn.edu\/wp-rest\/wp\/v2\/posts\/176308\/revisions"}],"predecessor-version":[{"id":176310,"href":"https:\/\/today.uconn.edu\/wp-rest\/wp\/v2\/posts\/176308\/revisions\/176310"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/today.uconn.edu\/wp-rest\/wp\/v2\/media\/176309"}],"wp:attachment":[{"href":"https:\/\/today.uconn.edu\/wp-rest\/wp\/v2\/media?parent=176308"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/today.uconn.edu\/wp-rest\/wp\/v2\/categories?post=176308"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/today.uconn.edu\/wp-rest\/wp\/v2\/tags?post=176308"},{"taxonomy":"magazine-issue","embeddable":true,"href":"https:\/\/today.uconn.edu\/wp-rest\/wp\/v2\/magazine-issues?post=176308"},{"taxonomy":"author","embeddable":true,"href":"https:\/\/today.uconn.edu\/wp-rest\/wp\/v2\/coauthors?post=176308"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}