The lab-on-paper platform detects two SARS-CoV-2 genes that are highly conserved across variants. So even as the virus mutates, Liu\u2019s test will still be able to detect it. The pH-EVD test provides a simple binary response for patients: positive or not for the SARS-CoV-2 virus.<\/p>\n
Liu\u2019s tests work much like a PCR test, which is the gold-standard for SARS-CoV-2 detection and required in many situations, such as before international travel, some surgeries, and more recently, before gaining entry to some sports events.<\/p>\n
PCR tests are more reliable than at-home rapid tests, because PCR tests search for the presence of SARS-CoV-2 genetic material, allowing them to pick up on active viral molecules \u2013 as does Liu\u2019s test.<\/p>\n
Currently available rapid tests check for the presence of antigens against SARS-CoV-2. If an infected person has not generated enough antigens when they take the test, they could get a false negative result. This is a potentially big problem, since someone could unknowingly spread the virus for days before a rapid test will detect the infection. These tests may also give false positive results if they pick up antigens after the infection passes, or from an old infection.<\/p>\n
While both Liu\u2019s devices and PCR tests are designed to detect SARS-CoV-2 genetic material, Liu\u2019s devices look more like a rapid test, since it is low-cost, easy-to-use, and much quicker than PCR tests.<\/p>\n Liu\u2019s pH-EVD device has 100% specificity, meaning it only detects SARS-CoV-2. This is on par with PCR tests. It also has 89% accuracy when it comes to detecting the presence or absence of the virus. Liu published these findings in Advanced NanoBIOMED Research<\/a>.<\/p>\n \u201cIn our lab, we have validated the performance of our technology using COVID-19 clinical samples,\u201d Liu says. \u201cIt is comparable to PCR technology.\u201d<\/p>\n It is difficult to compare this to currently available rapid tests, as there are many on the market. But one study<\/a> suggests the BinaxNOW test has a sensitivity of 87% in symptomatic individuals and 71% in asymptomatic individuals.<\/p>\n Liu\u2019s pH-EVD device includes a cellulose-based pH paper membrane, like many currently available rapid tests. It transports the liquid that contains the sample, obtained from a nasal swab. The pH paper produces color signals to indicate a positive or negative result in 40 minutes, compared to days for PCR results.<\/p>\n Patients would receive laboratory-level test results without having to go to a lab or clinic.<\/p>\n The pH-EVD device does not require any electricity to run, making it much more affordable than PCR tests. Instead, it uses heat generated automatically from chemical reactions to fuel the process. This allows the nucleic acids to amplify at a constant temperature. This amplification is what enables the technology to pick up on the viral genes.<\/p>\n This platform can also be applied for diagnosing other viruses such as HIV, influenza viruses, or high-risk HPV by modifying which genes it selects for.<\/p>\n The lab-on-paper diagnostic platform uses CRISPR technology to detect the SARS-CoV-2 genes. As a diagnostic tool, CRISPR locates the genes of interest and clips them. This clip produces an observable signal, which on Liu\u2019s platform appears as fluorescence filling in on the paper to indicate a positive result. The device also tests for a \u201chousekeeping\u201d gene. This gene will mark the paper to indicate the test was done properly with a high-quality sample.<\/p>\n \u201cI think, in the near future, ideally, infectious disease diagnosis will be done at home and in small clinics,\u201d Liu says. \u201cThis technology can adapt for home-based COVID-19 diagnosis or other disease diagnosis. If it\u2019s successfully commercialized, I think there\u2019s a really broad market.\u201d<\/p>\n Liu has filed a strong portfolio of patent applications on diagnostics related technologies with UConn Technology Commercialization Services.<\/a> One of his technologies was successfully exclusively licensed in February 2021. UConn is actively looking for a company that can commercialize the technology and any interested parties are encouraged to contact: \u00a0techcomm@uconn.edu.<\/a><\/p>\n <\/p>\n “UConn\u2019s Technology Commercialization Services \u00a0works with innovators, entrepreneurs, investors, and industry partners to transform UConn discoveries into products, companies, and jobs that benefit society and fuel economic development. Through a coordinated approach between tech transfer, licensing, and startup teams, TCS provides services that enable success for faculty, business, Connecticut, and beyond.”<\/em><\/p>\n The Department of Biomedical Engineering is a shared department with the UConn School of Dental Medicine, School of Medicine, and School of Engineering.<\/em><\/p>\n Follow UConn Research on <\/em>Twitter<\/em><\/a> & <\/em>LinkedIn<\/em><\/a>.<\/em><\/p>\n","protected":false},"excerpt":{"rendered":" A lab-on-paper platform that uses genetic markers to detect the virus even as it mutates <\/p>\n","protected":false},"author":147,"featured_media":180914,"comment_status":"closed","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"_acf_changed":false,"_crdt_document":"","wds_primary_category":0,"wds_primary_series":0,"wds_primary_attribution":0,"footnotes":""},"categories":[1866,2213,2256,2076,2166,1868,2364,2235,179],"tags":[],"magazine-issues":[],"coauthors":[2277],"class_list":["post-180894","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-engr","category-coronavirus","category-innovation","category-research","category-sdm","category-meds","category-technology-commercialization","category-today-homepage","category-uconn-health"],"pp_statuses_selecting_workflow":false,"pp_workflow_action":"current","pp_status_selection":"publish","acf":[],"publishpress_future_action":{"enabled":false,"date":"2026-05-03 05:31:28","action":"change-status","newStatus":"draft","terms":[],"taxonomy":"category","extraData":[]},"publishpress_future_workflow_manual_trigger":{"enabledWorkflows":[]},"_links":{"self":[{"href":"https:\/\/today.uconn.edu\/wp-rest\/wp\/v2\/posts\/180894","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/today.uconn.edu\/wp-rest\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/today.uconn.edu\/wp-rest\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/today.uconn.edu\/wp-rest\/wp\/v2\/users\/147"}],"replies":[{"embeddable":true,"href":"https:\/\/today.uconn.edu\/wp-rest\/wp\/v2\/comments?post=180894"}],"version-history":[{"count":10,"href":"https:\/\/today.uconn.edu\/wp-rest\/wp\/v2\/posts\/180894\/revisions"}],"predecessor-version":[{"id":181149,"href":"https:\/\/today.uconn.edu\/wp-rest\/wp\/v2\/posts\/180894\/revisions\/181149"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/today.uconn.edu\/wp-rest\/wp\/v2\/media\/180914"}],"wp:attachment":[{"href":"https:\/\/today.uconn.edu\/wp-rest\/wp\/v2\/media?parent=180894"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/today.uconn.edu\/wp-rest\/wp\/v2\/categories?post=180894"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/today.uconn.edu\/wp-rest\/wp\/v2\/tags?post=180894"},{"taxonomy":"magazine-issue","embeddable":true,"href":"https:\/\/today.uconn.edu\/wp-rest\/wp\/v2\/magazine-issues?post=180894"},{"taxonomy":"author","embeddable":true,"href":"https:\/\/today.uconn.edu\/wp-rest\/wp\/v2\/coauthors?post=180894"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}![\"\"](\"https:\/\/today.uconn.edu\/wp-content\/uploads\/2022\/01\/UConn-Today-updated-300x231.png\")