UConn’s Proteomics and Metabolomics Facility Gives Faculty a Complete Cellular Picture

Vials in a mass spectrometer
UConn's Proteomics and Metabolomics Facility houses multiple mass spectrometers and is directed by experts in mass spectrometry. (Carson Stifel/UConn Photo)

The words “proteomics” and “metabolomics” are more than just tongue-twisters. These important scientific disciplines can be just as complex to understand as they are to pronounce. Proteomics is defined as the large-scale study of proteins, and metabolomics is the scientific study of chemical processes involving metabolites.

In order to identify and quantify proteins, peptides, and small molecule metabolites, powerful mass spectrometry equipment is required. Faculty at the University of Connecticut can tap into these key resources at the Proteomics and Metabolomics Facility (PMF), which houses multiple mass spectrometers and is directed by experts in mass spectrometry.

To study proteins and metabolites, you need to be able to confidently identify each with certainty in the midst of complex mixtures of many molecules displaying similar characteristics.  Due to the molecular diversity present in most organisms, this can be a daunting task. At the same time, a simple molecular identification is not typically sufficient for most projects.  It is also important to simultaneously and accurately measure the amount of each molecule present in a given sample type. This allows you to understand how these molecules change over time or across different conditions in biological systems.

The PMF represents UConn’s first entry into providing proteomics services in over a decade, and is the Storrs campus’ first ever dedicated proteomics lab. Notably, within just over a year of opening its doors, the PMF has already served nearly 50 principal investigators between UConn Storrs and UConn Health spanning a wide variety of disciplines including chemistry, engineering, pharmacy, molecular biology, and dentistry among others.  Administratively, the PMF lives within the Center for Open Research Resources and Equipment (COR²E).

Peptides and metabolites enter the mass spectrometer using electrospray ionization.
Peptides and metabolites enter the mass spectrometer using electrospray ionization. (Carson Stifel/UConn Photo)

Most researchers who need mass spectrometry for their studies have not been previously trained to use this sophisticated instrumentation. That is precisely why facility director Jeremy Balsbaugh, Ph.D. provides guidance and training to prospective users of the PMF.

“One of the main focuses of this facility is the interaction between the researcher and myself so that we find answers to proteomics questions quickly and affordably,” says Balsbaugh. “I strive to keep open communication with researchers at each step of the project so that the data received comes quickly and is understandable to people who are not familiar with proteomics methods. It is really important to me that researchers understand the results of these experiments.”

Balsbaugh has years of experience in mass spectrometry. He received his Ph.D. in bioanalytical chemistry at the University of Virginia using mass spectrometry to study proteins and their post-translational modifications, and completed a postdoctoral fellowship focused on studying protein structure-function relationships using mass spectrometry at the University of Colorado at Boulder. After his fellowship, he led a mass spectrometry core facility at the University of Colorado at Boulder, using mass spectrometry to identify a vast array of different analytes.

“Many UConn researchers have been heavily focused on genomics, but having a well-established proteomics facility allows scientists to gain information about the proteins that are actually present instead of being limited strictly to knowledge of the genes that have been transcribed. This facility allows us to add one more component of understanding to the complete picture of what is happening in cells,” says Balsbaugh.

Many recent research projects have benefited from the use of this facility. UConn Health professor Dr. Pramod K. Srivastava has been working to identify peptide antigens that are expressed on the surface of cells for recognition by T cells. It is notoriously difficult to identify these sequences, and the use of mass spectrometry equipment available at the Proteomics and Metabolomics Facility has made this study possible. In addition, Sarah Reed from the Department of Animal Science is utilizing the facility for a large-scale quantitative proteomics experiment. Reed’s team is monitoring protein changes in sheep to determine how maternal diet affects protein expression in muscle.

Signs suggest that there will be many more exciting new projects to come; to date, nearly 30 UConn Storrs and UConn Health labs have submitted grant proposals for projects that are dependent on complex analyses in the PMF.

Balsbaugh encourages prospective users of the facility to reach out to him via email. He is available to assist researchers at every stage of their projects, from experiment design to execution.

“Proteomics and metabolomics are complex. We typically need to design the experiment together in order to get the best answer in the shortest amount of time with the highest success rate on the first attempt.”

Balsbaugh can be reached by email at jeremy.balsbaugh@uconn.edu and more information about PMF can be found at proteomics.uconn.edu

 

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